Price list (pdf)

DNA sequence reactions done by KIGene 

• template and primer concentrations

• plasmid DNA or PCR fragment?

• size of the template

• primer sequence

Material needed:

Purified PCR fragments          

•  5-50ng depending on length (~2 ng/100bp) in a volume of 5µl or less

Plasmid DNA                   

• 0.2µg-1.0µg depending on size (~0.2µg/5kb) in a volume of 10µl or less

Primer                                   

• 5pmol/reaction in a volume of 2µl or less

Sequencing of large clones (BAC, PAC and lambda) per reaction

• Plasmid DNA - 1.0µg
• Primer - 30pmol
• The total volume of plasmid and primer can not exceed 12µl.

We stock the following unlabelled primers:

BGH-R: 5'-TAG AAG GCA CAG TCG AGG-3'
CMV-F: 5'-CGC AAA TGG GCG GTA GGC GTG-3'
M13 F (-20), "standard": 5'-GTA AAA CGA CGG CCA GTG-3'
M13 F (-40): 5'-GTT TTC CCA GTC ACG AC-3'
M13 R (-27), "standard": 5'-CAG GAA ACA GCT ATG AC-3'
M13 R (-48): 5'-AGC GGA TAA CAA TTT CAC AC-3'
PGEX-3': 5'-CCG-GGA GCT GCA TGT GTC AGA GG-3'
PGEX-5': 5'-GGG-CTG GCA AGC CAC GTT TGG TG-3'
Sp6 Promoter: 5'-ATT TAG GTG ACA CTA TAG-3'
T3 Promoter: 5'-ATT AAC CCT CAC TAA AGG-3'
T7-EEV: 5'-ATG TCG TAA TAA CCC CGC CCC G-3'
T7 Promoter: 5'-AAT ACG ACT CAC TAT AGG-3'
T7 Reverse: 5'-TAG TTA TTG CTC AGC GGT G-3'
T7 Terminator: 5'-GCT AGT TAT TGC TCA GCG G-3'

When sending the material by post, use padded envelopes and wrap the tubes in Kleenex or similar to ensure they will not be damaged during the postal handling. Please use Parafilm over the lids.

Updated by Annika Eriksson Sept 28, 2011.