|
Automated fluorescent scanning detection of DNA fragments
is a very useful technology for mutation detection including
SSCP, microsatellite analysis including linkage mapping,
AFLP analysis, gene expression profiling and screening for loss of heterozygosity. "Hands-on" service
The facilty houses several different instruments that can be used for fragment analysis (ABI 3730 PRISM DNA Analyzer) and SNP analysis (ABI OpenArray Genotyping Platform, ABI 7900HT instrument and PSQ96MA). The equipment can be used by those who have sufficient knowledge and experience. The facility provides start up theoretical and technical assistance. Each user reserves a date and time for occupying the instrument by a booking system.
"Hands-off" service
Fragment analysis samples that will be analyzed on the ABI3730 instrument, should be supplied to us as fluorescently
labeled, pooled PCR products in 96-wells plates. The concentration of products need to be optimized by the user. The user can bring their own internal size standards or it will be provided by KIGene. Each
user must book an entire run (a minimum of 48 samples),
as each run is unique.
You can label different DNA fragments with up to four
different color fluorescent dyes. A fifth color is
reserved for the internal lane size standard that allows precise size calling by providing a means to normalize mobility during the run. We are using DS-33 (6-FAM, VIC, NED, PET and LIZ for the size standard) or DS-30 (6-FAM, HEX, NED and ROX for the size standard). We recommend the use of DS-33!
You will receive the resulting files through a FTP
based transferring system, which requires a FTP account (please contact us and we will set it up without charge), or by your e-mail.
Contact us:
Please, contact us at kigene@mmk.ki.se
for more information.
More information about genotyping at KIGene:
http://www.cmm.ki.se/en/Resources/Core-facilities/KI-Gene/
Updated
by Annika Eriksson
May, 2011.
|
